This course will cover the fundamentals of light microscopy, including different light paths, contrast techniques, principles of fluorescence, imaging capabilities, and confocal microscopy. It will be followed by hands-on training on PSF measurement, multi-dimensional image acquisition, 3D reconstruction, etc. Participants will gain practical experience using advanced imaging modalities, including widefield, point scanning, and spinning disk confocal microscopes. The hands-on sessions will be supplemented with individual exercises, assignment submissions, and discussions to address any questions
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